ebioscience 16 0038 85 ucht Search Results


cd3e (t-cell marker)(ucht1)  (Biotium)
99
Biotium cd3e (t-cell marker)(ucht1)
Cd3e (T Cell Marker)(Ucht1), supplied by Biotium, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ebioscience+16+0038+85+ucht/CD3e+(T-Cell+Marker)(UCHT1)/custom%40bnc042473-100%4038625017
Average 99 stars, based on 1 article reviews
cd3e (t-cell marker)(ucht1) - by Bioz Stars, 2026-09
99/100 stars
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cd3 epsilon antibody  (NSJ Bioreagents)
99
NSJ Bioreagents cd3 epsilon antibody
Cd3 Epsilon Antibody, supplied by NSJ Bioreagents, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ebioscience+16+0038+85+ucht/CD3+epsilon+Antibody/custom%40v3760%4030893608
Average 99 stars, based on 1 article reviews
cd3 epsilon antibody - by Bioz Stars, 2026-09
99/100 stars
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cd3 antibody  (NSJ Bioreagents)
99
NSJ Bioreagents cd3 antibody
Cd3 Antibody, supplied by NSJ Bioreagents, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ebioscience+16+0038+85+ucht/CD3+Antibody/custom%40f52219%4034960191
Average 99 stars, based on 1 article reviews
cd3 antibody - by Bioz Stars, 2026-09
99/100 stars
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anti-cd3 (cat. 555329/14-0038-82, clone ucht1)  (Becton Dickinson)
90
Becton Dickinson anti-cd3 (cat. 555329/14-0038-82, clone ucht1)
Anti Cd3 (Cat. 555329/14 0038 82, Clone Ucht1), supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ebioscience+16+0038+85+ucht/anti+cd3/10__1080_slash_2162402x__2017__1362529-98-6-17
Average 90 stars, based on 1 article reviews
anti-cd3 (cat. 555329/14-0038-82, clone ucht1) - by Bioz Stars, 2026-09
90/100 stars
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irradiated  (Rad Source Technologies)
99
Rad Source Technologies irradiated
Irradiated, supplied by Rad Source Technologies, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ebioscience+16+0038+85+ucht/RS+2000+X-Ray+Irradiator/us12312403-1887-73-81
Average 99 stars, based on 1 article reviews
irradiated - by Bioz Stars, 2026-09
99/100 stars
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cd161 (dx12; apc  (Becton Dickinson)
90
Becton Dickinson cd161 (dx12; apc
(A) Density plots demonstrating the gating strategy for MAIT cells in 1 healthy Haitian donor: left panel is gated on live CD3+ cells, and right panel is gated on live CD3+MR1-5-A-RU/MeG <t>tetramer+CD161++.</t> (B) Relative abundance of MAIT cell subsets among tetramer+CD161++ cells after 15 hours of rest (blue) or 5-A-RU/MeG activation (red) in 32 healthy Haitian donors. Same donors assessed in B–I. Same color coding applies to C and D. Data in B–D and F–I represent mean ± SD. (C) Abundance of MAIT cells among T cells. (D) Abundance of MAIT cells within their respective T cell subset. (E) Contour plots demonstrating the gating strategy for MAIT cell CD69 staining, a T cell activation marker, at rest or after 5-A-RU/MeG (left panels). Both plots are gated on live, CD3+, tetramer+CD161++. The right panel demonstrates CD69 staining in MAIT cell subsets at rest (blue), after 5-A-RU/MeG activation (red), or after treatment with neutralizing αMR1 antibody for 1 hour prior to 5-A-RU/MeG activation (green). Same color coding applies to F and G. (F) CD25 and (G) PD-1 staining in MAIT cell subsets. (H) GzB and (I) IFNγ measured by intracellular staining in the resting (blue), 5-A-RU/MeG (red), and anti-CD3/CD28 (purple) conditions in MAIT cell subsets. Groups were compared by 2-tailed unpaired t test with a significance level of P < 0.05. Asterisks above stimulated conditions indicate statistical significance compared with resting. Asterisks above the αMR1 condition indicate statistical significance compared with the 5-A-RU/MeG-stimulated condition. *P < 0.05, **P < 0.005, ***P < 0.0005, ****P < 0.0001; 5-A-RU, 5-amino-6-(D-ribitylamino)uracil; MeG, methylglyoxal; GzB, granzyme B.
Cd161 (Dx12; Apc, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ebioscience+16+0038+85+ucht/cd161++dx12+antibody/pmc06237486-411-24-27
Average 90 stars, based on 1 article reviews
cd161 (dx12; apc - by Bioz Stars, 2026-09
90/100 stars
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pd-1 (eh12.1; bv650  (Becton Dickinson)
90
Becton Dickinson pd-1 (eh12.1; bv650
(A) Density plots demonstrating the gating strategy for γδ T cells in 1 healthy Haitian donor: left panel is gated on live cells, and right panel is gated on live CD3+TCRγδ+ cells. (B) TCRγδ staining in CD4+ T cells (left) with IGRA stratification (right) in community controls (green) and household contacts (purple). Color coding applies to all panels. Data in B–F represent mean ± SD. (C) Relative abundance of TCRγ subsets within their respective T cell subsets (left) with IGRA stratification (right). <t>(D)</t> <t>PD-1</t> staining in CD4+ γδ T cells after 15 hours of rest (left) or anti-CD3/CD28 stimulation (right). Groups were compared by 2-tailed unpaired t test in A–D with significance level of P < 0.05. CD4+ γδ T cell PD-1 staining before or after anti-CD3/CD28 activation in IGRA+ community controls (E) and contacts (F). Groups were compared by 2-tailed paired t test in E and F with significance level of P < 0.05. *P < 0.05, **P < 0.005, ****P < 0.0001. IGRA, IFNγ release assay
Pd 1 (Eh12.1; Bv650, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ebioscience+16+0038+85+ucht/annexin+v/pmc06237486-411-43-46
Average 90 stars, based on 1 article reviews
pd-1 (eh12.1; bv650 - by Bioz Stars, 2026-09
90/100 stars
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jurkat cells antibody rabbit monoclonal phospho tyr 1000 cell signaling technology rrid ab 2687925  (Cell Signaling Technology Inc)
95
Cell Signaling Technology Inc jurkat cells antibody rabbit monoclonal phospho tyr 1000 cell signaling technology rrid ab 2687925
(A) Density plots demonstrating the gating strategy for γδ T cells in 1 healthy Haitian donor: left panel is gated on live cells, and right panel is gated on live CD3+TCRγδ+ cells. (B) TCRγδ staining in CD4+ T cells (left) with IGRA stratification (right) in community controls (green) and household contacts (purple). Color coding applies to all panels. Data in B–F represent mean ± SD. (C) Relative abundance of TCRγ subsets within their respective T cell subsets (left) with IGRA stratification (right). <t>(D)</t> <t>PD-1</t> staining in CD4+ γδ T cells after 15 hours of rest (left) or anti-CD3/CD28 stimulation (right). Groups were compared by 2-tailed unpaired t test in A–D with significance level of P < 0.05. CD4+ γδ T cell PD-1 staining before or after anti-CD3/CD28 activation in IGRA+ community controls (E) and contacts (F). Groups were compared by 2-tailed paired t test in E and F with significance level of P < 0.05. *P < 0.05, **P < 0.005, ****P < 0.0001. IGRA, IFNγ release assay
Jurkat Cells Antibody Rabbit Monoclonal Phospho Tyr 1000 Cell Signaling Technology Rrid Ab 2687925, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ebioscience+16+0038+85+ucht/Stat2+Rabbit+mAb/10__7554_slash_elife__64251-284-74-80
Average 95 stars, based on 1 article reviews
jurkat cells antibody rabbit monoclonal phospho tyr 1000 cell signaling technology rrid ab 2687925 - by Bioz Stars, 2026-09
95/100 stars
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monoclonal mouse cd90-pe 5e10  (Becton Dickinson)
90
Becton Dickinson monoclonal mouse cd90-pe 5e10
(A) Density plots demonstrating the gating strategy for γδ T cells in 1 healthy Haitian donor: left panel is gated on live cells, and right panel is gated on live CD3+TCRγδ+ cells. (B) TCRγδ staining in CD4+ T cells (left) with IGRA stratification (right) in community controls (green) and household contacts (purple). Color coding applies to all panels. Data in B–F represent mean ± SD. (C) Relative abundance of TCRγ subsets within their respective T cell subsets (left) with IGRA stratification (right). <t>(D)</t> <t>PD-1</t> staining in CD4+ γδ T cells after 15 hours of rest (left) or anti-CD3/CD28 stimulation (right). Groups were compared by 2-tailed unpaired t test in A–D with significance level of P < 0.05. CD4+ γδ T cell PD-1 staining before or after anti-CD3/CD28 activation in IGRA+ community controls (E) and contacts (F). Groups were compared by 2-tailed paired t test in E and F with significance level of P < 0.05. *P < 0.05, **P < 0.005, ****P < 0.0001. IGRA, IFNγ release assay
Monoclonal Mouse Cd90 Pe 5e10, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ebioscience+16+0038+85+ucht/cd90+antibody/pm37149858-43-15-22
Average 90 stars, based on 1 article reviews
monoclonal mouse cd90-pe 5e10 - by Bioz Stars, 2026-09
90/100 stars
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monoclonal mouse cd31-pe-cy7 wm59  (Becton Dickinson)
90
Becton Dickinson monoclonal mouse cd31-pe-cy7 wm59
(A) Density plots demonstrating the gating strategy for γδ T cells in 1 healthy Haitian donor: left panel is gated on live cells, and right panel is gated on live CD3+TCRγδ+ cells. (B) TCRγδ staining in CD4+ T cells (left) with IGRA stratification (right) in community controls (green) and household contacts (purple). Color coding applies to all panels. Data in B–F represent mean ± SD. (C) Relative abundance of TCRγ subsets within their respective T cell subsets (left) with IGRA stratification (right). <t>(D)</t> <t>PD-1</t> staining in CD4+ γδ T cells after 15 hours of rest (left) or anti-CD3/CD28 stimulation (right). Groups were compared by 2-tailed unpaired t test in A–D with significance level of P < 0.05. CD4+ γδ T cell PD-1 staining before or after anti-CD3/CD28 activation in IGRA+ community controls (E) and contacts (F). Groups were compared by 2-tailed paired t test in E and F with significance level of P < 0.05. *P < 0.05, **P < 0.005, ****P < 0.0001. IGRA, IFNγ release assay
Monoclonal Mouse Cd31 Pe Cy7 Wm59, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ebioscience+16+0038+85+ucht/cd31fitc+antibody/pm37149858-43-25-32
Average 90 stars, based on 1 article reviews
monoclonal mouse cd31-pe-cy7 wm59 - by Bioz Stars, 2026-09
90/100 stars
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percp-cy5.5 mouse anti-human cd196 (ccr6) 11a9  (Becton Dickinson)
90
Becton Dickinson percp-cy5.5 mouse anti-human cd196 (ccr6) 11a9
(A) Density plots demonstrating the gating strategy for γδ T cells in 1 healthy Haitian donor: left panel is gated on live cells, and right panel is gated on live CD3+TCRγδ+ cells. (B) TCRγδ staining in CD4+ T cells (left) with IGRA stratification (right) in community controls (green) and household contacts (purple). Color coding applies to all panels. Data in B–F represent mean ± SD. (C) Relative abundance of TCRγ subsets within their respective T cell subsets (left) with IGRA stratification (right). <t>(D)</t> <t>PD-1</t> staining in CD4+ γδ T cells after 15 hours of rest (left) or anti-CD3/CD28 stimulation (right). Groups were compared by 2-tailed unpaired t test in A–D with significance level of P < 0.05. CD4+ γδ T cell PD-1 staining before or after anti-CD3/CD28 activation in IGRA+ community controls (E) and contacts (F). Groups were compared by 2-tailed paired t test in E and F with significance level of P < 0.05. *P < 0.05, **P < 0.005, ****P < 0.0001. IGRA, IFNγ release assay
Percp Cy5.5 Mouse Anti Human Cd196 (Ccr6) 11a9, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ebioscience+16+0038+85+ucht/3d12+antibody/pm32160527-435-12-13
Average 90 stars, based on 1 article reviews
percp-cy5.5 mouse anti-human cd196 (ccr6) 11a9 - by Bioz Stars, 2026-09
90/100 stars
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Image Search Results


(A) Density plots demonstrating the gating strategy for MAIT cells in 1 healthy Haitian donor: left panel is gated on live CD3+ cells, and right panel is gated on live CD3+MR1-5-A-RU/MeG tetramer+CD161++. (B) Relative abundance of MAIT cell subsets among tetramer+CD161++ cells after 15 hours of rest (blue) or 5-A-RU/MeG activation (red) in 32 healthy Haitian donors. Same donors assessed in B–I. Same color coding applies to C and D. Data in B–D and F–I represent mean ± SD. (C) Abundance of MAIT cells among T cells. (D) Abundance of MAIT cells within their respective T cell subset. (E) Contour plots demonstrating the gating strategy for MAIT cell CD69 staining, a T cell activation marker, at rest or after 5-A-RU/MeG (left panels). Both plots are gated on live, CD3+, tetramer+CD161++. The right panel demonstrates CD69 staining in MAIT cell subsets at rest (blue), after 5-A-RU/MeG activation (red), or after treatment with neutralizing αMR1 antibody for 1 hour prior to 5-A-RU/MeG activation (green). Same color coding applies to F and G. (F) CD25 and (G) PD-1 staining in MAIT cell subsets. (H) GzB and (I) IFNγ measured by intracellular staining in the resting (blue), 5-A-RU/MeG (red), and anti-CD3/CD28 (purple) conditions in MAIT cell subsets. Groups were compared by 2-tailed unpaired t test with a significance level of P < 0.05. Asterisks above stimulated conditions indicate statistical significance compared with resting. Asterisks above the αMR1 condition indicate statistical significance compared with the 5-A-RU/MeG-stimulated condition. *P < 0.05, **P < 0.005, ***P < 0.0005, ****P < 0.0001; 5-A-RU, 5-amino-6-(D-ribitylamino)uracil; MeG, methylglyoxal; GzB, granzyme B.

Journal: JCI Insight

Article Title: Mucosal-associated invariant and γδ T cell subsets respond to initial Mycobacterium tuberculosis infection

doi: 10.1172/jci.insight.121899

Figure Lengend Snippet: (A) Density plots demonstrating the gating strategy for MAIT cells in 1 healthy Haitian donor: left panel is gated on live CD3+ cells, and right panel is gated on live CD3+MR1-5-A-RU/MeG tetramer+CD161++. (B) Relative abundance of MAIT cell subsets among tetramer+CD161++ cells after 15 hours of rest (blue) or 5-A-RU/MeG activation (red) in 32 healthy Haitian donors. Same donors assessed in B–I. Same color coding applies to C and D. Data in B–D and F–I represent mean ± SD. (C) Abundance of MAIT cells among T cells. (D) Abundance of MAIT cells within their respective T cell subset. (E) Contour plots demonstrating the gating strategy for MAIT cell CD69 staining, a T cell activation marker, at rest or after 5-A-RU/MeG (left panels). Both plots are gated on live, CD3+, tetramer+CD161++. The right panel demonstrates CD69 staining in MAIT cell subsets at rest (blue), after 5-A-RU/MeG activation (red), or after treatment with neutralizing αMR1 antibody for 1 hour prior to 5-A-RU/MeG activation (green). Same color coding applies to F and G. (F) CD25 and (G) PD-1 staining in MAIT cell subsets. (H) GzB and (I) IFNγ measured by intracellular staining in the resting (blue), 5-A-RU/MeG (red), and anti-CD3/CD28 (purple) conditions in MAIT cell subsets. Groups were compared by 2-tailed unpaired t test with a significance level of P < 0.05. Asterisks above stimulated conditions indicate statistical significance compared with resting. Asterisks above the αMR1 condition indicate statistical significance compared with the 5-A-RU/MeG-stimulated condition. *P < 0.05, **P < 0.005, ***P < 0.0005, ****P < 0.0001; 5-A-RU, 5-amino-6-(D-ribitylamino)uracil; MeG, methylglyoxal; GzB, granzyme B.

Article Snippet: PBMCs were stained with Zombie Red Fixable Viability Dye (BioLegend, catalog 423109) and antibodies to CD3 (UCHT1; Alexa700; eBioscience; Thermo Fisher Scientific, catalog 16-0038-81), CD161 (DX12; APC; BD Biosciences, catalog 550968), CD69 (FN50; BV421; BioLegend, catalog 310929), CD25 (BC96; BV711; BioLegend, catalog 302635), PD-1 (EH12.1; BV650; BD Biosciences, catalog 564104), CD4 (SK3; PerCPefluor or BV650; eBioscience; Thermo Fisher Scientific, catalog 46-0047-41, or BD Biosciences, catalog 563876) and CD8 (SK1; APC-H7; BD Biosciences, catalog 560179), Vγ24Jα18 (6B11; BV510; BioLegend, catalog 342917) and TCR γδ (B1; FITC; BioLegend, catalog 331207), and MR1-5-OP-RU tetramers (NIH tetramer core facility; PE or BV421) for 15 minutes at room temperature in the dark.

Techniques: Activation Assay, Staining, Marker

(A) Density plots demonstrating the gating strategy for γδ T cells in 1 healthy Haitian donor: left panel is gated on live cells, and right panel is gated on live CD3+TCRγδ+ cells. (B) TCRγδ staining in CD4+ T cells (left) with IGRA stratification (right) in community controls (green) and household contacts (purple). Color coding applies to all panels. Data in B–F represent mean ± SD. (C) Relative abundance of TCRγ subsets within their respective T cell subsets (left) with IGRA stratification (right). (D) PD-1 staining in CD4+ γδ T cells after 15 hours of rest (left) or anti-CD3/CD28 stimulation (right). Groups were compared by 2-tailed unpaired t test in A–D with significance level of P < 0.05. CD4+ γδ T cell PD-1 staining before or after anti-CD3/CD28 activation in IGRA+ community controls (E) and contacts (F). Groups were compared by 2-tailed paired t test in E and F with significance level of P < 0.05. *P < 0.05, **P < 0.005, ****P < 0.0001. IGRA, IFNγ release assay

Journal: JCI Insight

Article Title: Mucosal-associated invariant and γδ T cell subsets respond to initial Mycobacterium tuberculosis infection

doi: 10.1172/jci.insight.121899

Figure Lengend Snippet: (A) Density plots demonstrating the gating strategy for γδ T cells in 1 healthy Haitian donor: left panel is gated on live cells, and right panel is gated on live CD3+TCRγδ+ cells. (B) TCRγδ staining in CD4+ T cells (left) with IGRA stratification (right) in community controls (green) and household contacts (purple). Color coding applies to all panels. Data in B–F represent mean ± SD. (C) Relative abundance of TCRγ subsets within their respective T cell subsets (left) with IGRA stratification (right). (D) PD-1 staining in CD4+ γδ T cells after 15 hours of rest (left) or anti-CD3/CD28 stimulation (right). Groups were compared by 2-tailed unpaired t test in A–D with significance level of P < 0.05. CD4+ γδ T cell PD-1 staining before or after anti-CD3/CD28 activation in IGRA+ community controls (E) and contacts (F). Groups were compared by 2-tailed paired t test in E and F with significance level of P < 0.05. *P < 0.05, **P < 0.005, ****P < 0.0001. IGRA, IFNγ release assay

Article Snippet: PBMCs were stained with Zombie Red Fixable Viability Dye (BioLegend, catalog 423109) and antibodies to CD3 (UCHT1; Alexa700; eBioscience; Thermo Fisher Scientific, catalog 16-0038-81), CD161 (DX12; APC; BD Biosciences, catalog 550968), CD69 (FN50; BV421; BioLegend, catalog 310929), CD25 (BC96; BV711; BioLegend, catalog 302635), PD-1 (EH12.1; BV650; BD Biosciences, catalog 564104), CD4 (SK3; PerCPefluor or BV650; eBioscience; Thermo Fisher Scientific, catalog 46-0047-41, or BD Biosciences, catalog 563876) and CD8 (SK1; APC-H7; BD Biosciences, catalog 560179), Vγ24Jα18 (6B11; BV510; BioLegend, catalog 342917) and TCR γδ (B1; FITC; BioLegend, catalog 331207), and MR1-5-OP-RU tetramers (NIH tetramer core facility; PE or BV421) for 15 minutes at room temperature in the dark.

Techniques: Staining, Activation Assay, Release Assay

(A) Density plots demonstrating the gating strategy for MAIT cells in 1 healthy Haitian donor: left panel is gated on live CD3+ cells, and right panel is gated on live CD3+MR1-5-A-RU/MeG tetramer+CD161++. (B) Relative abundance of MAIT cell subsets among tetramer+CD161++ cells after 15 hours of rest (blue) or 5-A-RU/MeG activation (red) in 32 healthy Haitian donors. Same donors assessed in B–I. Same color coding applies to C and D. Data in B–D and F–I represent mean ± SD. (C) Abundance of MAIT cells among T cells. (D) Abundance of MAIT cells within their respective T cell subset. (E) Contour plots demonstrating the gating strategy for MAIT cell CD69 staining, a T cell activation marker, at rest or after 5-A-RU/MeG (left panels). Both plots are gated on live, CD3+, tetramer+CD161++. The right panel demonstrates CD69 staining in MAIT cell subsets at rest (blue), after 5-A-RU/MeG activation (red), or after treatment with neutralizing αMR1 antibody for 1 hour prior to 5-A-RU/MeG activation (green). Same color coding applies to F and G. (F) CD25 and (G) PD-1 staining in MAIT cell subsets. (H) GzB and (I) IFNγ measured by intracellular staining in the resting (blue), 5-A-RU/MeG (red), and anti-CD3/CD28 (purple) conditions in MAIT cell subsets. Groups were compared by 2-tailed unpaired t test with a significance level of P < 0.05. Asterisks above stimulated conditions indicate statistical significance compared with resting. Asterisks above the αMR1 condition indicate statistical significance compared with the 5-A-RU/MeG-stimulated condition. *P < 0.05, **P < 0.005, ***P < 0.0005, ****P < 0.0001; 5-A-RU, 5-amino-6-(D-ribitylamino)uracil; MeG, methylglyoxal; GzB, granzyme B.

Journal: JCI Insight

Article Title: Mucosal-associated invariant and γδ T cell subsets respond to initial Mycobacterium tuberculosis infection

doi: 10.1172/jci.insight.121899

Figure Lengend Snippet: (A) Density plots demonstrating the gating strategy for MAIT cells in 1 healthy Haitian donor: left panel is gated on live CD3+ cells, and right panel is gated on live CD3+MR1-5-A-RU/MeG tetramer+CD161++. (B) Relative abundance of MAIT cell subsets among tetramer+CD161++ cells after 15 hours of rest (blue) or 5-A-RU/MeG activation (red) in 32 healthy Haitian donors. Same donors assessed in B–I. Same color coding applies to C and D. Data in B–D and F–I represent mean ± SD. (C) Abundance of MAIT cells among T cells. (D) Abundance of MAIT cells within their respective T cell subset. (E) Contour plots demonstrating the gating strategy for MAIT cell CD69 staining, a T cell activation marker, at rest or after 5-A-RU/MeG (left panels). Both plots are gated on live, CD3+, tetramer+CD161++. The right panel demonstrates CD69 staining in MAIT cell subsets at rest (blue), after 5-A-RU/MeG activation (red), or after treatment with neutralizing αMR1 antibody for 1 hour prior to 5-A-RU/MeG activation (green). Same color coding applies to F and G. (F) CD25 and (G) PD-1 staining in MAIT cell subsets. (H) GzB and (I) IFNγ measured by intracellular staining in the resting (blue), 5-A-RU/MeG (red), and anti-CD3/CD28 (purple) conditions in MAIT cell subsets. Groups were compared by 2-tailed unpaired t test with a significance level of P < 0.05. Asterisks above stimulated conditions indicate statistical significance compared with resting. Asterisks above the αMR1 condition indicate statistical significance compared with the 5-A-RU/MeG-stimulated condition. *P < 0.05, **P < 0.005, ***P < 0.0005, ****P < 0.0001; 5-A-RU, 5-amino-6-(D-ribitylamino)uracil; MeG, methylglyoxal; GzB, granzyme B.

Article Snippet: PBMCs were stained with Zombie Red Fixable Viability Dye (BioLegend, catalog 423109) and antibodies to CD3 (UCHT1; Alexa700; eBioscience; Thermo Fisher Scientific, catalog 16-0038-81), CD161 (DX12; APC; BD Biosciences, catalog 550968), CD69 (FN50; BV421; BioLegend, catalog 310929), CD25 (BC96; BV711; BioLegend, catalog 302635), PD-1 (EH12.1; BV650; BD Biosciences, catalog 564104), CD4 (SK3; PerCPefluor or BV650; eBioscience; Thermo Fisher Scientific, catalog 46-0047-41, or BD Biosciences, catalog 563876) and CD8 (SK1; APC-H7; BD Biosciences, catalog 560179), Vγ24Jα18 (6B11; BV510; BioLegend, catalog 342917) and TCR γδ (B1; FITC; BioLegend, catalog 331207), and MR1-5-OP-RU tetramers (NIH tetramer core facility; PE or BV421) for 15 minutes at room temperature in the dark.

Techniques: Activation Assay, Staining, Marker